Other

Part:BBa_I716310:Design

Designed by: Vincent Parker   Group: iGEM07_Berkeley_UC   (2007-10-25)


T7-RBS-OtsbBA-TT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 2415
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 2415
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2400
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 2415
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 2415
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3502


Design Notes

Digest I716307 (BglII, Bsa1, 970, 3443, L) Sub into I716306 (BamHI, Bsa1, Alwn1, 548, 1119, 549, L) Product is I716310

Source


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

References